Zoolyx NV

H353 Coat Colour Saddle tan vs black-and-tan


During the last decades, a large number of scientific publications have described the genetic principles of coat colour and coat variation. Coat colours and coat variations are influenced by many hereditary factors. The DNA-tests are based on physiological effects in the body, in which the production and distribution of pigments result in many coat colour variants. In several cases, the coat colour of an animal may only be decided using DNA-tests.

The hnRNP associated with lethal yellow gene (RALY gene) defines whether tan points or saddle tan is expressed in Basset Hounds and Pembroke Welsh Corgi dogs. Black and tan colour is characterized by light colour on the muzzle, above the eyes (tan points) and on the undersides of the dog on otherwise dark coat. Saddle tan resembles black and tan colour but the lighter areas are expanded leaving usually only the back to have dark patch. Saddled tan dogs are usually born black-and-tan and the black recedes as the dog grows. The coat colour is further complicated by the interaction with the E-locus, K-locus, A-locus and a yet unidentified gene. In order for the saddle tan pattern or tan points to be expressed, the dog needs to have at least one copy of the E or Em allele at the E-locus, two copies of the ky allele at the K-locus and one or two copies of the at allele at the A-locus. The Coat Colour Saddle tan vs black-and-tan test (H353) tests for the genetic status of the RALY gene. The RALY gene has two variants (alleles). The allele WT is dominant and causes the saddle tan coat colour. Only when the dog has two copies of the recessive allele dup the coat colour is black-and-tan. The saddle tan coat colour is present in a limited number of dog breeds including some of the terriers, scent hounds and herding dogs. In breeds that have only tan point dogs and no saddled tan dogs, the tan pointed dogs can have any genotype for the RALY gene. This suggests that more complex interactions are behind tan points in breeds that are not able to express saddle tan.

Test specific information

Since 2015, two brands have been developed. CombiGen® is mainly directed at veterinarian applications, whereas CombiBreed® is mainly directed at breeders and/or owners.
Detailed information about Coat Colours and Coat Variation is presented at www.combibreed.com.


Most coat colours and coat types are usually visible directly after birth.

Turnaround time

The Turnaround Time (TAT) depends on various factors, such as the shipment time of your sample to the test location, the test method(s) and whether the tests are performed completely or partially by a Partner Lab or Patent owner.

The TAT of tests performed at our facilities is normally 10 working days after receipt of the sample at the testing laboratory (VHL, VHP or Certagen). For tests performed by a Partner Laboratory (so-called "partner lab test") or patent owner, the TAT is at least 20 working days after receipt of your sample. Because the shipment time to our Partner Labs or patent owner may vary due to factors we cannot influence, the mentioned 20 working days are therefore an estimate.

Sometimes it is necessary to re-run your sample. We call this a retest. In that case, the TAT will of course be extended.

Location of disease or trait

Genetic factors influencing coat colours and coat types are usually visible on the outside of an individual. Several factors may be hidden by the external variation.

Breed dependence

This DNA test is available for the following breeds: Basset, Pembroke Welsh Corgi. Additional information is available in the Frequently Asked Questions (FAQ).

Sample type

For this DNA test we accept the following materials: Blood EDTA, Blood Heparin, Tissue, Semen, Swab. Please contact Dr. Van Haeringen Laboratorium if you wish to submit other material as listed.


Coat colours and coat types are based on many genetic factors. For each factor, a separate test result will be returned.


Various genetic factors influencing coat colour and coat types are inherited in a dominant or recessive mode. Coat colours are influenced by a large number of genetic factors.

Severity of Disease

Factors influencing coat colour and coat types are usually not related to diseases.

Code H353

Coat Colour Saddle tan vs black-and-tan

€ 50,94 (Incl. 21% VAT)
€ 42,10 (Excl. VAT)